| | Category | ME | L27 | Comparison of Method for Hemoglobin Quantification: Electrical |
| | Conductivity vs |
| | Abstract | The purpose of this study is to create an alternative method for analyzing |
| | hemoglobin that is accurate, efficient, simple, safer and more cost |
| | effective than the current gold standard (colorimetry). Blood samples were |
| | collected in EDTA tubes from consenting individuals. Dilutions of blood in |
| | de-ionized water were made and I used an electrical conductivity probe to |
| | measure iron ions from the samples collected. The ADVIA 2120i |
| | (colorimetric method), was then used to measure the same diluted blood |
| | samples in normal saline. The data collected was compared with samples |
| | of blood run on ADVIA 2120i.My project demonstrates that the conductivity |
| | method is a simple and effective method for analyzing hemoglobin levels. |
| | The results show that my method is comparable to the colorimetric method |
| | in estimating hemoglobin levels. The conductivity method also has the |
| | ability to quantify hemoglobin at higher dilutions, but this was not seen in |
| | colorimetry. |
| | |
| | In conclusion my method using the conductivity probe to measure Fe++ |
| | ions in the |
| | de-ionized aqueous solution is just as effective as the colorimetric method. |
| | It is also economical and does not use toxic substances such as |
| | potassium cyanide, which is present in hemolyzing solution used in the |
| | colorimetric method. Therefore the conductivity technique is a method that |
| | can potentially be employed in areas that can't afford a colorimeter or it |
| | can be used as a small hand held device at home for patients that need to |
| | regularly check their hemoglobin. |
| | |
| | Bibliography | 1. McPherson R, Pincus M: Henry's Clinical Diagnosis and Management by |
| | Laboratory Methods, |
| | 21st Edition, Saunders Elseviers, 457-467,2007 |
| | 2. Ackerman P: Electronic Instrumentation in the Clinical Laboratory: |
| | Boston, Little Brown & Co., |
| | 1972 p140 |
| | 3. Bentley SA, Johnson A, Bishop CA: A Parallel Evaluation of Four |
| | Automated Hematology |
| | Analyzers. Am J Clin Path 1993; 100: 626-632. |
| | 4. Wintrobe MM. 'Clinical Hematology' 9th Edition. Philadelphia: Lea and |
| | Febiger; 1993, pp 123 |
| | 5. Hemoglobin estimation by the revolutionary colorimetric method of |
| | Kennedy, |
| | JAMA. 1944;126(2):95-96. |